ABSTRACT
Objective To investigate the association of DNA methyltransfcrase 3B (DNMT3B) promoter region single nucleo tide polymorphism (SNP)-149C＞T and-579G＞T with genetic susceptibility of esophageal cancer (EC) of Han population in Jiangsu Suqian.Methods Gcnotypes of the-149C＞T and 579G＞T locus in DNMT3B promoter region were examinedby polymerase chain reaction with restriction fragment length polymorphism (PCR RFLP) in 246 esophageal cancer patients and 240 healthy controls.Results In-149C＞T site,the distributions of genotypes TT compared with CT in the EC group and the controls were no significant difference (x2 =0.089,P＞0.05).In-579G＞T site,the distributions of genotypes TT compared with GT+GG in the EC group and the controls were no significant difference (x2 =0.649,P＞0.05).When strat ified by age and gender,there was no significant difference in the EC group and the controls in both two sites (x2 =0.044～0.876,all P＞0.05).Conclusion The DNMT3B promoter region-149C＞T and-579G＞T were not associated with the genetic susceptibility of esophageal cancer.
ABSTRACT
Background Selective laser trabeculoplasty(SLT)has been widely accepted for the treatment of open-angle glaucoma.However,there are few reports about its application in treating angle-closure glaucoma so far.Objective This study was to clinically evaluate the safety and efficacy of selective laser trabeculoplasty for primary angle-closure glaucoma with opened anterior angle follow-up surgery.Methods A case-observational design was adopted in this study.A total of 33 eyes from 23 angle-closure glaucomatous patients with opened 180° angle were enrolled.All the patients understood this trial and written informed consent was obtained prior to the medical treatment.The average preoperative intraocular pressure(IOP)and number of medication were(23.07±4.01)mmHg and 1.52±0.66,respectively.A frequency doubled Q-switched Nd:YAG laser was used to irradiate the trabecular meshwork with 70-80 laser spots over 180°.The same preoperative anti-glaucoma drugs were topically administered during the follow-up period.Visual acuity,IOP,slit lamp examination,gonioscopy and the coefficient of outflow facility were regularly assessed and performed after laser surgery.Results The IOP values of the 33 eyes were(18.78±4.87)mmHg,(18.83±3.95)mmHg,(19.78±4.48)mmHg,(19.63±3.68)mmHg,(19.11±3.36)mmHg,and(21.17±3.14)mmHg 1 day,l week,and 1,3,6,9 months after operation,showing significant decline in comparison with preoperation(P＜0.01).The mean drug uses were 1.63±0.85,1.23±1.01,1.25±0.98,1.21±1.02,1.22±0.86,and 1.69±0.53 at the same time point after operation,respectively,and significant differences were found 1 week,1,3,6 months in postoperation in comparison with preoperation(P =0.020,0.026,0.020,0.031).Ciliary congestion and aqueous flare naturally disappeared 1 week after surgery.The coefficients of outflow facility were 0.11±0.09 and 0.23±0.15,respectively at pretreatment and 3 months after SLT with a statistically significant difference (P=0.002).Conclusions Selective laser trabeculoplasty is effective and safe for angle-closure glaucoma.However,it is essential to have 1/2 circle of anterior chamber open for SLT in angle-closure glaucoma.